Pathological experiment TIMM dyeing

TIMM method is a ionic state, loose binding Or zinc ion dyeing in chelate state, easy to light, electron microscope observation.

It is capable of displaying common methods for heavy metal distribution in tissue cells. The end of the boss fiber is a region having a high zinc content, so it can be used in this method to show the distribution of moss fiber. Experimental process:

(1) Sloth stickers Acid on the slide of the polyxereoniic acid treated slide, -20 is stored. (2) take it out to dry, about 10 minutes.

(3) Pour the TIMM developer into the repair box, placed in 26

The water bath or the constant temperature shaker is stained with 90-150 min, and the dyeing must be under the conditions of exposure. conduct.

(4) After staining, the slice is rinsed several times with distilled water, which can be reset to Niki dyeing or not reset. (5) Dry, neutral resin sealing film. Service Description (1) The customer provides: paraffin slice.

(2) The company provides: pictures, results and analysis. (3) Experiment cycle: 2 weeks.