Pathological experiment Masson dyeing

Service introduction Masson is dyed, the collagen fiber is green (green is dyed), The muscle fibers were red (dyed by acidic magenta and Lichunhong), which was related to the size of the anion dye molecule and the permeability of the tissue. The red blood cells have small permeability of the anion dyes, the muscle fibers and the cytoplasm, and the collagen fibers have large permeability, so the red blood cells are dyed red, and the muscle fibers are dyed with cytoplasm. It is red, while collagen fibers are green by macromolecule anion dyes.

Experimental process

(1) paraffin sections Wax to the water.

(2) 5-10 min of Nucleoving solution with Regaud Swaphumi or Weigert Suijing.

(3) is washed with water and washed distilled water.

(4) 5-10 min with Masson Lichikin acidic.

(5) dipped in a 2% aqueous aqueous solution of ice acetic acid.

(6) Differentiation 3-5 min in a 1% phosphorly boss acid solution.

[123) without water washing (8) Take 0.2% aqueous Icevetate aqueous solution

[ 123] (9) 95% alcohol, waterless alcohol, transparent in xylene, neutral gum is sealed. Service Description

(1) The customer provides: paraffin slice.

(2) Company provides: basic experimental steps, pictures, analysis results.

(3) Experimental cycle: about 2 weeks, the specific needs according to experimental content.