Pathological experiment Nishi staining

Nissl dyed liquid is blue purple, Commonly used to display basic neural structures of the brain or spinal cord. Nissl is small and large, indicating that nerve cell synthesis proteins are more functional; the number of NISSL small bodies will be reduced even disappeared when nerve cells are damaged. The active ingredient of Nissl dyeing liquid is an alkaline dye tar purple (CRESYL VIOLET), also known as the phenoli, which can be combined with RNA or DNA, can be stained with ribosome on crude endoplasm, or also dyed Nuclear nucleus. After dyeing, the cell body exhibits a mottled blue purple dye. Alkaline dyes such as Thionine (sulfur); Tolridine Blue (toluene blue) can also be used.

Experimental process

(1) Sample treatment

(2) Nikite staining: 2-10 minutes, distilled water (2 times each time)

(3) Dehydration, transparent, sealing [ 123] Service Description

(1) Customer Provide: Sample . (2) The company provides: pictures, results and analysis.

(3) Experiment cycle: 2 weeks.