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Transwell Cell invasion experiment

Time:2017-12-02 Click:1248

TRANSWELL cell invasion experiment

Cell invasion experimental purpose: the effect of chemokine or other nutrients on cell invasion

Transwell Cell invasion experiment

Principle: The cell species into the upper chamber, chemokine, etc., and the cells will run in the lower room of the nutrients. Unlike the migration experiment, the invasion experiments need to be laid a layer of matrix glue on the small chamber polycarbonate film. Used to mimic the extracellular matrix in the body, the cytokines will enter the next room, first secrete matrix metalloproteinase (MMPS) to degrade the matrix glue, and can pass the polycarbonate film. The amount of cells entering the next chamber can reflect the abnormality of tumor cells.

Experimental material instrument: ultra-clean workbench, CO2 incubator, biological inverted microscope

TRANSWELL Cell invasion experiment

Content and method: [123

1. Cell seed species in the upper chamber, chemokine, etc., etc.

2. After the culture time, the calculation is observed or determined. Related Physiotherapy Information

Customers provide experimental information: 1, experimental information (providing):

2, the experimental material is provided

(1) Sample material:

a host cell strain, cell culture conditions in a growing condition [ 123] (2) Reagents:

TRANSWELL chambers need customers to provide or entrust our purchase, if there is a drug, the drug concentration is provided

Submit: Submit an experiment report (experimental reagent and equipment, experimental process, results analysis, raw data, cell picture, etc.)

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