Shanghai TUNEL test experiment
Shanghai TUNEL test experiment
When apoptosis occurs, some DNA [ 123] Internal resembrase, these endozymes cut off genome between nuclear versus DNA [ 123] . perform electrophoresis detection, which can be found [ [123 . Genomic When breaking, exposed [ 3 u0026 RSQUO; -OH can be deoxycodide transferase [123 Catalytic Catalyzed Container [ (FITC) Tag [123 DUTP (Fluorescein-DUTP) , so that detection can be carried out by a fluorescent microscope or flow cytometry, this is [ 123] TUNEL (TDT-Mediated Dutp Nick-End Labeling) The principle of apoptosis was detected. TUNEL detection (paraffin slice Removes the appropriate fresh tissue block to fix the Among polymethylene or neutral formaldehyde, the volume ratio of formaldehyde and tissue block is 10: 1 and [ 123] 4 [123 Celsius storage transport;
TUNEL[123 Removed fresh tissue should immediately put the ultra-low temperature refrigerator Or in liquid nitrogen, use dry ice, fixed slices should be in
-80 -20 Save, and is used to detect as soon as possible TUNEL detection (cell climb) Cell climb 4% Multi-formaldehyde degree fixed 30 min
, change [123 Buffer
Save transportation, used to detect as soon as possible; [123 Fluorescence method needs to take an apoptosis fluorescent map, take a place in situ dye, and then consolidate the layer, and the test is three fluorescent pictures.