Shanghai cell migration experiment

Shanghai Cell Migration Experiment Service introduction Cell migration refers to cellular cells in receiving a migration signal or feeling some The movement generated after the gradient of the substance. Cell migration is an extension of cellular head pseudo-sufficient, new adhesion, and the cellular end of the cell is contracted in time and space. Cell migration is one of the basic functions of normal cells. It is the physiological process of normal growth and development of the body, and is also a motion form of living cells. Embryonic development, angiogenesis, wound healing, immune response, inflammatory response, atherosclerosis, cancer metastasis, etc., are involved in cell migration. Experiments for detecting cell migration capabilities include scratches experiments and Transwell experiments.

Second,

Shanghai cell migration experiment Experimental principle

Cell scratches experiments are simple to determine cell migration movement and repair Method of capabilities, in vitro culture dishes or plate-cultured single-layer adapter cells, the central region of the central portion is removed with a trace gun head or other hard object, and the central portion of the cells are removed, then the cells are continued to cultivate cells to experiment settings. Time is a simple and easy way to detect cell movement, and the experimental cost can be used to detect invasive metastasis of tumor cells. The basic principle of Transwell is to place the Transwell chamber into the culture plate, referred to as the upper chamber, referred to as a lower chamber, and the upper and lower micro carbonate film is separated by a polycarbonate film, and the upper layer culture solution is added in the upper chamber. Add The lower culture solution. The cell species is in the upper chamber. Since the film has permeability, the ingredient in the lower medium can affect the cells in the upper chamber, so that the ingredients in the lower culture fluid can be investigated to cell growth, exercise, and the like.

3,

Scratch experiment

1 It is uniformly scratched on the 6-well plate;

2. Add cells in the well;

3. Two-day gun head is tied to the horizontal scratches behind

4. Wash the cells under the PBS, cultivate different times, sampling, taking photos;

[123 5. Data Processing: Each time point cell overall migration distance u003d time distance length -0 hour distance;

6. Result statistical analysis.

TRANSWELL experiment

1. Using unburned Matrigel glueThe Transwell Chamber was used for experiments; 2. Preparation of cell suspensions;

3. Inoculated cells;

4. The number of cells passed;

5. Results statistical analysis.

Four,

Cell migration experiment The customer provides

Cell strain (frozen storage or cultured cells),The drug, drug treatment method and concentration. Five, the company provides

Basic experimental steps, pictures, data analysis results, surplus drugs.

Experimental cycle: about 2-3 weeks, specifically, according to cell growth and experimental content.