TIMM dyeing pathological experiment
TIMM dyeing experiment Method for chemical positioning of zinc ionic tissue, silver dyeing method (NEO-TIMM) sulfide method, NEO-TIMM method is to ionic, loose binding or chelation Zinc ion staining in the state, facilitating light, observation of electron microscopy.
TIMM dyeing method is a common method of displaying the heavy metal distribution of tissue cells. The end of the boss fiber is a region having a high zinc content, so it can be used in this method to show the distribution of moss fiber.
TIMM tissue chemical staining ( TIMM dyeing experiment ): ): 1. Sliced u200b u200battached Multi-bleeding treated slides. (-20 u0026 deg; save)
2. take it out to dry (10 minutes).
3. Pour the TIMM developer into the repair box, put it in a water bath or constant temperature shaker in a temperature of 26 ° C, staining 90-150 min, staining under the conditions of exposure conduct. 4. After dyeing, the slice is rinsed with distilled water several times, and can be reset to Niki dyeing or not reset.
5. Dry, neutral resin seal film.
TIMM liquid configuration:
A: Protective glue: 30 g of Arabic glue is dissolved in 60 ml of deionized water (thoroughly dissolved). For two days, if there is impurities, double-layer gauze can be filtered. When the glue is not urgent, the freezing is stored in the plastic bottle to save for at least 6 months.
B: Citrate buffer: 2.55 citric acid u0026 bull; H2O plus 2.35 sodium citrate u0026 bull; 2H2O, ion water to 10 ml. C: Restorer: 0.85 g of phenylene phenol (hydroquinone) is dissolved in 15 ml of deionized water.
D: Silver ion supply: 0.11 g of silver (silver nitrate) is added to 15 ml of deionized water. Silver lactate is sensitive to light, so this solution should be protected.
A B C solution was carefully mixed, and the D solution was added immediately before it was used. It is more appropriate to do eight to ten films at a time.
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Experimental cycle: 3-4 weeks