Explorators sequencing experiment services
First, the exon sequencing experimental service introduction Examite group sequencing (exome-seq) is High-throughput sequencing of the directional enrichment genomic DNA can be copied in a relatively low fee. In 2009, the emergence of exon group capture tools, allowing exon group to sequencing this technology quickly red. So far, more than 10,000 exon groups have been sequenced.
There are now many companies launched exon group capture reagents, including Roche Nimblegen, Agilent, Illumina, and near China. These methods are universal, and the researchers of the Genetic School of Stanford University School of Medicine have compared the three mainstream exons measurement platforms in the market. The results are on "Nature Biotechnology ". The researchers analyzed the same human blood samples using Agilent, Illumina and Nimblegen's exon group sequencing platform. Their results show that the NIMblegen platform is * a platform using a high-density overlapping probe, although less genome areas, the amount of sequence required is small when the sensitive detection is small. In the case where the 80m sequencing data is obtained, NIMBLEGEN has a 97% target sequence reaches 10X or more sequencing depth, while other products are only 90%. The probe of Agilent and Illumina can capture more numerous variations at low densities. In addition, Illumina also captured an unrelated area, which is the NIMBLEGEN and Agilent platforms that cannot be targeted. The researchers also compared the exon group sequencing and all genome sequencing (WGS) of the same sample, and showed that the exon group sequence can detect a small variation of WGS missed.
In addition to the three mainstream platforms, new products aspects of the exon group are continuously launched, and the choice of researchers will be wider. Roche Nimblegen is about to launch a new generation of exon liquid capture products. This new product will capture 64M genomic sequences, including exons and miRNA, which contains the same 2.1M high-density probe as other NIMblegen liquid captures to be efficient, uniform, specific, oriented. In order to make researchers more economical and more economical to use directional exon group sequencing, Illumina provides Truseq Exme EnrichmentKit, a solution type sequence capture method. The exon range from the human genome is separated by hybridization selection. Truseq EXME ENRICHMENTKit provides an exon group coverage as so far.
Second, Outer dominant sequencing experiment service analysis project
[ 123] Standard Analysis Project
1. Data quality control and screening2. Compared with the reference genome comparison, the sequencing depth READS distribution and coverage statistics
3.SNP, Small INDEL detection[ 123]
Analysis item
somatic mutant site screening (cancer) [123
Note (adjacent to gene regions and functional annotations, proximity domain notes, adjacent plants, variation types of coding region internal site; variation site Conservative detection, variation site risk assessment) Data set picture type statistics (multi-sample)
[ 123]
High-frequency variant site / gene statistics (multi-sample)
GWAS analysis (large sample amount)