All genetic group weight sequencing experiment
All genetic group reproduction is known species for genome sequences. Individuals perform genome sequencing, and a method of differential analysis at an individual or group level. Based on all genometric sequencing techniques, we can quickly perform seed resource census screening, find a large number of genetic variants, and achieve genetic evolution analysis and important selection of important selection. As sequencing costs, reselling, the relegation of the sequencing cost reduction and the known genome sequence, the relegation has become one of the rapid and effective methods in the study of animal and plant breeding. Second,
All genetic group weight sequencing experimental serviceOperation process [ 123] The genomic DNA is extracted and randomly interrupts, and the DNA fragment of the desired length is recovered, and the joint is added to the CLUSTER preparation, and the machine sequencing. Sequencing both ends of the insertion fragment using a two-terminal (paired-end), in addition to obtaining sequence information, it is conducive to mining of large fragment structural variation.
Third,Indicator Evaluation
Sequencing depth is the evaluation An important indicator of the sequencing amount, the increase in sequencing is advantageous for the rise of genome coverage and simultaneously reduces the sequencing error rate. The genome coverage and sequencing error rate control are obtained when the sequencing individual level is sequentially sequencing. In terms of group, 3-5X can be obtained
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