Shanghai microRNA target gene verification experiment

MicroRNA target gene verification experiment Service introduction MicroRNA's functional study of target genes is one of transcriptional regulation studies Important direction. The use of luciferase reporter genes to verify that MicroRNA's target effect is a common experimental technology.

Second, Shanghai

microRNA target gene verification experiment

Service process

1,

3, the target sequence clone

[ 123] 4, positive clonation identification 5, transfection

6, fluorescerase detection

Third, customers provide

Name, Sequence, Sequence or GeneBank Accession Number

] 1, double fluorescene enzyme report gene wild-type carrier, mutant vector; 2, experimental report: detailed steps, carrier map, carrier sequencing file, double fluorescene detection;

V. Shanghai

MicroNA target gene verification experiment

Service list

Project

cycle

miRNA, target Gene prediction 2 working days Targeting 3 u0026 RSQUO; UTR diblactin enzyme wild carrier ( u0026 lt; 2.5 kb) 10 working days [ 123] Targeting 3 u0026 RSQUO; UTR Double Lucomase Mutant Vector (A Situation) 10 Work Day miRNA expression vector or MIMIC and double fluorescene support co-transfection 20 working days Dibluozyme detection 5 working days Total 47 working days