Shanghai Qikini transfection experiment

Qiki gene transfection experiment Service introduction Cell transfection refers to exogenous molecules such as DNA, RNA is introduced into eukaryotic cells. At present, the method of introducing foreign substance into cell is mainly divided into three categories: 1, liposome (or liposome substitute) transfection; 2, electric rotation; 3, viral infection. Transfection techniques can be divided into transient transfection and stable transfection

The functional study of genes can be divided into loss-of-function and gain-of-function, that is, expressed Genes and the means of inhibiting expression of endogenous genes to study a biological function of a gene. Conventional transfection techniques can be divided into two categories, one is instantaneous transfection, and one is stable transfection (* transfection).

Instant transfection

Shanghai Zhen Nuclear gene transfection experiment

Temperature-mediated transfection gene transfection techniques by liposome, introducing target genes into cells, generally 48 hours after transfection The target gene can be expressed in intracellularization. According to different experimental purposes, the detection of target gene expression can be performed in 48 hours. The function of the target gene and its expression protein in a certain cell (observation can be performed in a short time, but the effect will be lost quickly)

Shanghai Qikin gene Transfection experiment

Serve 1, receive plasmid, cell

2, cell culture

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3, transfection

4, photo

5, efficiency identification

1, constructing a good foreign gene carrier, or is constructed by the company (the company needs to provide the purpose of the gene, the species, Sequence or gene an accession number).

2, the cell line to be transfected (1 u0026 Times; 106 cells, can be passed, multiplied time is less than 48 hours), and the conditions of cell culture conditions. 3, if the expression of the target gene is required, the corresponding antibody is required or purchased by the company.

Delivery content

1, constructing a good transient transfection cell. 2, experimental report: Detailed experimental steps and related exogenous gene expression analysis.

Construction of stabilizing transfection cell line

Service introduction

Stable transfection, is the transfected plasmid DNA integrates into host cell chromosomes, allowing host cells to express their purpose and proteins for a long time. The target cells are required to screen or apply a high-transfected efficiency based on instantaneous transfection. According to the resistance symbols contained in different gene carriers, the cellular passage is selected to obtain a cell line that can stably express the gene. .

1, receiving plasmid, cell

[ 123] 2, cell culture

3, transfection 4, antibiotic screening

5. A monoclonal cell line

7, detection

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1. Constructs a good exogenous gene carrier, or optionally constructed by company (price reference carrier construction).

2, the cell line to be transfected (1 u0026 Times; 106 cells, can be passed, multiplied time is less than 48 hours), and the conditions of cell culture conditions.

3, provide corresponding antibody

Delivery content

[123 ] 1. Constructing a good stable expression cell line.

2, experimental report: detailed steps, related foreign gene expression analysis, etc.

Second, service list

project

[123 ] Remark Cycle

Instantaneous transfection + identification Identification method is Western Blot

10 working days Stable transfection + identification

Identification method is Western Blot

45 working days (adhesive cells)