Shanghai microRNA chip experiment
1, miRNA real-time quantitative PCR (Real-Time Quantitative PCR) detection
u0026 EMSP; u0026 EMSP; use mature real-time quantitative PCR technology Accurate and quickly quantitatively detect the expression of MicroRNA in various tissues, primary cells and cell lines, high sensitivity, high experimental cycle and high specificity, and experimental results are shown in Figures 1 and 2.
Fig. 1 u0026 EMSP; EMSP; amplification curve of miRNA and internal parametrin (U6 snRNA)
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Fig. 2 u0026 EMSP; melted curve of miRNA and internal parametrin (U6 snRNA) (instrument is Roche LC 480)
2, MicroNA Array Shanghai MicroRNA chip experiment
u0026 EMSP; u0026 EMSP; uses of miRNA expression profiles integrated on 384-well plates to detect humans, rats or mice's miRNA expression profiles, only one working day can be covered Integrated data set for Sanger miRbase. The principle of TAQMAN probe is used, and the results are accurate and reliable, and the sample does not need to be repeated.
3, Specific type of disease miRNA expression detectionu0026 EMSP; u0026 EMSP; miRNA expression detection is important for monitoring the development of tumors and the effects of various treatment methods, as guided individual chemicals The selected biomarker has a large clinical application potential. We perform fluorescent quantitative detection for a variety of tumor-related iconic miRNA, such as liver cancer, gastric cancer, pancreatic cancer.
Service Number
u0026 Nbsp;
Sending sample requirements
6
), tissue ( u0026 GE; 300 mg), blood ( u0026 GE; 1 mL), serum ( u0026 GE; 1.5ml) sample material, total RNA ( u0026 GE; 20 u0026 Mu; L, concentration u0026 ge; 50 ng / u0026 MU; L).
Shanghai microRNA chip experiment success case
[123 ] miRNA has an important role in the process of cancer,Hospital customers study the difference in miRNA expression in case groups and control groups in order to confirm whether miRNA plays regulation during the mechanism.
MICRORNA differential expression quantification example
