Shanghai Expression Spectral Chip Experiment

Shanghai Expression Spectral Chip Experiment

Experimental Process: RNA extraction, then reverse into cDNA, in vitro transcriptional synthesis cDNA, firor, chip hybridization, washing, scanning.

Shanghai Expression Spectral Chip Experiment

Service Content: Professional Technical Services, unique inkjet in-situ synthesis technology, flexible customization plan, only genome or EST sequence, customers can make Your chip

Experiment Example

The signal value distribution graph shows the chip probe Distribution statistics. The abscissa represents the probe expression interval, and the ordinate represents the inner probe statistic in the expression value; the legend is located above the left side of the histogram; each curve represents a number of probes of a chip in different expression value intervals. The weight of the signal value distribution curve is, the higher the reliability of the chip experiment. The project chip is very reliable.

The clustering diagram shows the aggregation of the sample and the differential gene at the expression value level. Red indicates that the signal value of the gene is relatively upregulated. The green representation of the gene's signal value is relatively downward, and the signal value of the gene is indicated, and the gray representation of the signal value of the gene is not detected; the illustration is at the top left of the clustering map; the upper tree structure is based on the signal value of the sample to hierarchical The tree structure obtained by the cluster, the left tree structure is the signal value of the differential gene to obtain a typical structure to obtain a tree structure. Two samples or genes adjacent in the tree structure indicate higher similarities.

The gene network diagram illustrates the signal transfer process between the differential gene between the organism or cell gene and the gene, providing a possible signal transmission path for molecular mechanisms. Green representative chip reduction gene: dot size and gene expression difference in the experimental group, the larger the dot, the larger the expression difference in the experimental group and the control group; red representation gene chip up-regulate gene: dot size and experimental group The difference in gene expression is correlated, the larger the dot, the larger the expression difference in the experimental group and the control group; the associated gene added by the gray represents the system.