Chromatin immunoassate precipitation experiment

Chromatin immunopetal precipitation experiment

Chromatin Immunoprecipitation Assay, Chip is a method of studying DNA and protein interactions in vivo environments, commonly used Study on the in vivo transcription factor binding sites and the like. The basic principle is to fix the protein-DNA complex in a living cell state, and then disconnected to chromatin fragments in the length range, and then precipitate this complex by immunological methods, specifically enriched the combination of protein. The DNA fragment is obtained by purifying and detection of the piezoelectric fragments, thereby obtaining information on proteins interacting with DNA.

1. Cell culture 2. Cell Formaldehyde crosslinking and ultrasonic crushing 3. Incubate in addition to hybrid and antibody 4. Check the effect of ultrasound crushing 5. Precision and cleaning of immunoreduges 6. Recycling of DNA samples 7. Detection analysis 8. Data analysis processing, finishing report [123

1. Immunite protein information, predicted transcription factor binding position Point information and other related research background 2. Sample amount information and detection requirements for specifically to test 3. CHIP-grade antibody delivery requirements information: 1. Cell sample: Each detection sample requires at least 1 * 10 ^ 7 cell quantity; transport: Transport: 75T cell culture flask, filled with fresh transportation or provide cytoprost, dry ice transport; it is recommended to provide cells from our company to the need. 2. Tissue sample fresh tissue samples, animal tissue u003e 100mg / like; plant tissue u003e 5g / like; tissue sample dry ice transportation. ※ The remaining special samples need to be concluded

     1. Original results data And analysis 2. Detailed experiment report, including complete experimental steps, reagent instrument information, etc.