Erotic gene transfection experiment

Transfer an exogenous gene (vectors) into the cell Inside, it has become an important means of studying genes and cellular functions. It is currently commonly used including small RNA intervention (RNAi) and exogenous expression vectors to transfer two means. Both exert genetic silencing and gene expression, in realization of genes Knockout, identification of the biological characteristics of the gene, study gene expression and silence to play an important role in studying the regulation mechanism of gene expression and the control mechanism of gene expression.

Erotic gene transfection experiment

Service introduction The target gene is introduced into the cell by liposome-mediated transfection, and the target gene is introduced into the cells, and the target gene can be in the cell. Internal expression. According to different experimental purposes, the detection of target gene expression can be performed in 48 hours. The function of the target gene and its expression protein in a certain cell (observation in a short time, but the effect will be lost quickly)

Service Flow

1, receive plasmid, cell 2, cell culture

3 4, take pictures

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Customer provides:

1, constructing a good foreign gene carrier, or carrier constructs by the company (providing the purpose of gene names, sequences, or Gene Accession Number). 2, the cell line to be transfected (1 u0026 Times; 106 cells, can be passed, multiplied time is less than 48 hours) and cells Description of culture conditions.

[1 3, if the expression of the target gene is required, the corresponding antibody is required or purchased by the company.

Delivery content

1. Construct a good transient transfection cell. 2, an experimental report: Detailed experimental steps and associated exogenous gene expression analysis.