1. Luciferase reporter gene detection service introduction Luciferase reporter gene system is a luciferin as a substrate to detect the activity of firefly fluorescene (Ireflyluciferase) activity Reporting system. Luciferase can catalyze Luciferin oxidation into Xyluciferin, in the process of...

First, the carrier construction service introduction vectorconstruction is transported to the receptor cells in the receptor cell. First, it is necessary to find a carrier that can enter the cells that can still be copied in the form of a foreign DNA section. The ideal carrier is a plasmid, in the...

First, in situ hybridization introduction In situ hybridization (IN SITU HBRDZATIO) is a nucleic acid probe with a labeled nucleic acid probe and tissue, cells, etc.The genetically bonded hybrids formed by the target gene in accordance with the base of the base, and then uses the detection...

First, the scanning electron microscope service introduction Scanning electron microscope, ie scanning electron microscope (SEM) , the sample is scanned by extremely narrow electron beam, and the electron beam and the sample are used. J uses a variety of effects, that is, the surface morphology of...

1. ROS detection service introduction Active oxygen ( reactive oxidative species, ros) refers to oxygen-containing free radicals related to oxygen metabolism in biology. The intermediate product having high activity in the form of the base form can cause oxidation of the cell membrane...

1. Transmission electron microscopy detection service introduction Transmission electron microscope, ie transmit electron microscope (TEM) , the electron beam is a light source, it can be seen that it cannot be seen under optical microscope. 0. 2 um ultrastructure, mainly used in ultrastructural...

First, the calcium ion detection service introduction Calcium ions play an important role in the physiological activities of cells, the changes of free calcium ion concentrations and cellular function, transmission of signals and their damage And we have close contacts. Calcium ions are a single...

[Determination of Cell Growth Curves] Master the principles and methods of drawing cell growth curves. Strusion of Cell Growth Curve] The growth curve is a common method of determining the number of cellular growth, and is an important indicator to determine cell viability. It is the basics of...

Experimental operation process: osteogeneous induced culture fluid equipped with induction: 1. Prepare a A-MEM culture solution containing 10% FBS; 2 In the amount of EM culture Add 5OUM Decreasic acid 10mm micro-glycerol 00m dexamethasone; 3, prepare 6-bonus culture plate, inoculated P4 cells in...