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363

07-23

Pathological experiment TIMM dyeing

TIMM method is a ionic state, loose binding Or zinc ion dyeing in chelate state, easy to light, electron microscope observation. It is capable of displaying common methods for heavy metal distribution in tissue cells. The end of the boss fiber is a region having a high zinc content, so it can be...

358

10-15

Pathological experiment Nishi staining

Nissl dyed liquid is blue purple, Commonly used to display basic neural structures of the brain or spinal cord. Nissl is small and large, indicating that nerve cell synthesis proteins are more functional; the number of NISSL small bodies will be reduced even disappeared when nerve cells are damaged....

757

01-25

Pathological experiment TUNEL detection

TUNELASE (TDT) -Mediated Dutp Nick End Labeling, end deoxycodide transferase mediated DUTP gap end marker assay), also known as the in situ tag method of DNA break. The breakage of chromosome DNA in apoptosis is a progressive stage of step, and the chromosome DNA is first decreased to 50-300 kB of a...

674

02-16

Cell experiment cycle detection

Flow cytometry (Flow Cytometry or Flow Cytomyter, FCM) Fluorescence Activated Cell Sorter, FACS is a new technique for determining radioactive chromosomes using laser and laminar flow chambers in the late 1960s. Cell cycle analysis was carried out by FCM by determining the amount of DNA in the cell....

485

06-20

RNA interference experiment

RNA interference ( RNA interfering, RNAi) phenomenon refers to a silencer process after genetically transcribed with a target gene sequence homologous double-stranded RNA (DSRNA) bows, small interference RNA Specific mediated mRNA degradation of the same sequence, blocking the corresponding gene...

786

01-04

EQ nuclear expression vector construction experiment

First, the service introduction Molecular clone provides a method of purifying and amplifying a particular DNA segment at a molecular level. Often contains a target gene, inserting them into a cloned carrier, forming a recombinant cloning carrier, forming a suitable host, then introducing a suitable...

1120

02-20

Slow virus construction

Slow virus construct: Lentivirus is one of the reverse transcriptional virus, which has the basic structure and characteristics of reverse transcription virus; integrated into a host cell genome, long-term expression, can be prepared for transgenic animals; but there is also different from reverse The...

1157

07-03

Double fluorescene report gene detection experiment

Experimental meaning 1. It can be widely referenced by target gene detection of miRNA, and it can be judged whether or not miRNA is combined with target genes, or it can quantify the inhibitory effect of miRNA to the purpose of interest. 2 Transcription factor ( transformer factor) There is a DNA...

414

09-26

Real-time fluorescent quantitative PCR experiment

Provides fast and efficient real-time fluorescence quantitative PCR (Real PCR) service, so-called real-time fluorescence quantitative PCR technology refers to the addition of a fluorescent group in a PCR reaction system, accumulating fluorescent signals.Monitor the entire PCR process in real time,...

572

09-01

Molecular cloning experiment

Introduction: Molecular cloning and plasmid vector construction service experimental technical services original authentic, quality assurance price concessions. Molecular cloning and plasmid vector construction services | Experimental technical services a PCZN1 plasmid + Arctic Express Host...