05-29
1. Cell culture: Take the number of growth cells, inoculated in 96-well plates in 96-well plates, 4x103 to 1x 105 cells per well, cultured to normalGrowth phase 2. Drug treatment: Various pharmaceutical treatment according to experimental needs 3. EDU mark: The EDU solution (reagent A) is diluted...
08-25
Experiments are divided into four main steps: 1. Cell laying HeLa S3 cells, density is 80% left and right, make the spread density at 50 % Or so, in order to meet the requirements of flow cytometry, the number of cells is greater than 1 x 10 groups. 2. Cell synchronization treatment: a)...
11-19
Reactive Oxygen Species, ROSs include superoxide radicals, hydrogen peroxide, and downstream product peroxide and hydroxylate, etc., involved in cell growth proliferation, developmental differentiation, aging and Dear and many physiological and pathological processes. The DHE active oxygen detection...
03-15
1, cell suspension preparation: Take a single-stage cultured cell in several days, digested with 0. 25% trypsin and Blowing into a single cell, retained with complete medium, and produced cell suspension. 2, Cell Formula: Dilute the cell suspension to a gradient multiple, inoculation with a gradient...
12-26
Ultra-clean workbench, CO2 incubator, biological inverted microscope, 6-well plate (other can, but feel 6 holes, size, size Moderately), Marker pen, straight ruler 20, microlifier head (sterilization), serum-free medium, PBS Cell scratches All can sterilize Sterilization, ruler and Marker pen in...
07-08
Flow cytometry is based on flow cytometry, physical phragens , Physiology, biochemical, immunization, genetic, molecular biological character and functional state, etc. The cells are identified, classified, quantitatively and separated. The immunomagnetic bead cell sisdom is specifically labeled with...
02-21
Certain chemical components in tissue sections or cell specimens with labeled specific antibodies The distribution and content are qualitatively, positioned or quantitative studies of tissue and cells, which referred to as immunohistochemistry or immunocyte chemochemistry technology. The...
05-03
Service introduction [ 123] The nature of easy being alkaline or acidic dyes is called basophysical and eosinophils, and the phenomenon of affinity for basic dyes and acidic dyes is called neutral. Cell acidic substances such as nuclear neat nucleus, adenocytes and neurocytes, the transparent...
06-17
Tianyou Star Red is a strong acidic anion dye Each molecule contains 6 sulfonic acid groups, which is easy to bind to the alkaline amino acid group of collagen. After dyeing, it is not faded, and the dye molecule is long-shaped, and the collagen is arranged in parallel. . When there is a polarized...
04-06
Service introduction Masson is dyed, the collagen fiber is green (green is dyed), The muscle fibers were red (dyed by acidic magenta and Lichunhong), which was related to the size of the anion dye molecule and the permeability of the tissue. The red blood cells have small permeability of the anion...