Immunohistochemical experiment Immunohistochemistry (IHC) was used to detect protein expression in formalin fixed, paraffin embedded (FFPE) tissue samples. Protein expression can be evaluated by color detection or fluorescence detection. Both detection systems are dependent on imperative antigenic...

Ingenic cell culture and separation service content 1. Prototype cells. 2 Separation of primary cells. 3. The cultivation and identification of primary and passage cells; after long-term experimental verification, the purity of various primary cells we cultivate reached more than 90%.4....

1. Cell culture: Take the number of growth cells, inoculated in 96-well plates in 96-well plates, 4x103 to 1x 105 cells per well, cultured to normalGrowth phase 2. Drug treatment: Various pharmaceutical treatment according to experimental needs 3. EDU mark: The EDU solution (reagent A) is diluted...

Experiments are divided into four main steps: 1. Cell laying HeLa S3 cells, density is 80% left and right, make the spread density at 50 % Or so, in order to meet the requirements of flow cytometry, the number of cells is greater than 1 x 10 groups. 2. Cell synchronization treatment: a)...

Reactive Oxygen Species, ROSs include superoxide radicals, hydrogen peroxide, and downstream product peroxide and hydroxylate, etc., involved in cell growth proliferation, developmental differentiation, aging and Dear and many physiological and pathological processes. The DHE active oxygen detection...

1, cell suspension preparation: Take a single-stage cultured cell in several days, digested with 0. 25% trypsin and Blowing into a single cell, retained with complete medium, and produced cell suspension. 2, Cell Formula: Dilute the cell suspension to a gradient multiple, inoculation with a gradient...

Ultra-clean workbench, CO2 incubator, biological inverted microscope, 6-well plate (other can, but feel 6 holes, size, size Moderately), Marker pen, straight ruler 20, microlifier head (sterilization), serum-free medium, PBS Cell scratches All can sterilize Sterilization, ruler and Marker pen in...

Flow cytometry is based on flow cytometry, physical phragens , Physiology, biochemical, immunization, genetic, molecular biological character and functional state, etc. The cells are identified, classified, quantitatively and separated. The immunomagnetic bead cell sisdom is specifically labeled with...

Flow cytometry (Flow Cytometry or Flow Cytomyter, FCM) Fluorescence Activated Cell Sorter, FACS is a new technique for determining radioactive chromosomes using laser and laminar flow chambers in the late 1960s. Cell cycle analysis was carried out by FCM by determining the amount of DNA in the cell....