485

06-20

RNA interference experiment

RNA interference ( RNA interfering, RNAi) phenomenon refers to a silencer process after genetically transcribed with a target gene sequence homologous double-stranded RNA (DSRNA) bows, small interference RNA Specific mediated mRNA degradation of the same sequence, blocking the corresponding gene...

786

01-04

EQ nuclear expression vector construction experiment

First, the service introduction Molecular clone provides a method of purifying and amplifying a particular DNA segment at a molecular level. Often contains a target gene, inserting them into a cloned carrier, forming a recombinant cloning carrier, forming a suitable host, then introducing a suitable...

1120

02-20

Slow virus construction

Slow virus construct: Lentivirus is one of the reverse transcriptional virus, which has the basic structure and characteristics of reverse transcription virus; integrated into a host cell genome, long-term expression, can be prepared for transgenic animals; but there is also different from reverse The...

1157

07-03

Double fluorescene report gene detection experiment

Experimental meaning 1. It can be widely referenced by target gene detection of miRNA, and it can be judged whether or not miRNA is combined with target genes, or it can quantify the inhibitory effect of miRNA to the purpose of interest. 2 Transcription factor ( transformer factor) There is a DNA...

414

09-26

Real-time fluorescent quantitative PCR experiment

Provides fast and efficient real-time fluorescence quantitative PCR (Real PCR) service, so-called real-time fluorescence quantitative PCR technology refers to the addition of a fluorescent group in a PCR reaction system, accumulating fluorescent signals.Monitor the entire PCR process in real time,...

572

09-01

Molecular cloning experiment

Introduction: Molecular cloning and plasmid vector construction service experimental technical services original authentic, quality assurance price concessions. Molecular cloning and plasmid vector construction services | Experimental technical services a PCZN1 plasmid + Arctic Express Host...

1094

02-23

FISH experiment

In situ hybridization With a complementary base sequence between the nucleic acid molecular single strands, radioactive or non-radioactive exogenous nucleic acids (ie, probes) are tested to tissue, cells or chromosomes.DNA or RNA complementary pair, combined into special- - nucleic acid hybrid...

514

12-17

Steady transcendal cell strain

Steady transform cell line Overgraph or silently expressed in host cells, the gene is stably expressed in the host cell, and then the functional study of the purpose gene is studied. Experiment Period and Delivery Standard 1. Experimental Period: 4-8 Week 2. Delivery Standard: Steady Cell...

369

10-30

Agarose gel electrophoresis experiment

Agarose gel electrophoresis: techniques such as agarose gel as media DNA in an external electric field. Experimental step Gelatin: Use electrophoretic buffer to formulate 1.0% agarose gel mixed by electrophoretic buffer: 2 u0026 mu; l - like buffer, 2 u0026 mu; l nucleic acid fluorescenceDyes,...

363

09-02

All gene synthesis experiment

The laboratory usually uses PCR and RT- PCR methods to acquire a gene fragment, but it is often unavailable because of specimens and templates, including . Today, we use chemical synthesis to artificially construct a gene fragment, namely: all gene synthesis splicing technology. Can serve the...