Shanghai Methically Test Experiment Service Project [123 1, methylation-Specific PCR (MSP) Treatment of genome DNA with bisulfite, and the methylated cytosine is converted to uracil. The methylated cytosine is unchanged; the primer for methylation and unoformat sequences are then designed to...

SNP (SINGLE NUCLETIDE POLYMORPHISM), ie mononucleotide polymorphism Sexuality is due to a single nucleotide change. In general, only two alleles are only two alleles, so it is also called a double allele. The frequency of SNP is relatively high in the human genome, and there is a multi-state site per...

Fluorescent quantitative PCR detection technology has been born to date has been more than 10 years The time, and its application has not been widely launched, the reason is not subject to the development of related instruments, reagents and technologies. Recently, especially in 2008, the instruments...

primer design and synthetic experiment. 1, [123 primer design and synthetic experiment. polymerase chain reaction, PCR technology It is a method of rapidly amplifying a particular gene or DNA sequence in vitro, so that the in vitro amplification method is also known. PCR technology has...

Steady strain screening experiment Steady transformation cell line (stabilizing expression cell line) refers to the continuous stability expression of a particular gene or interfere with specific gene expression in cells. The purpose of steady cell strain is integrated into the cell chromosome,...

over-expressing lentiviral packaging construct and test over-expressing lentivirus product, the customer to transfer the gene sequence of the CDS lentiviral vectors, The lentiviral particles of the transaction target gene were obtained by packaging; Lentivirus (LV) belongs to the reverse...

Electrophoresis is a commonly used laboratory technology that can be identified , Quantitatively and purify nucleic acid fragments. The sample is placed on the hole of the agarose or acrylamide gel and placed in an electric field to move the negatively charged nucleic acid. The shorter DNA fragment...

Shanghai fixed point mutation experiment If your gene or carrier is not your desired, or you need to do a key control for genes, components, etc. Functional research, you may need to do some mutations for its specific bitmap, or multiple sites do something to do. In general, there are different...

Chromatin immunopetal precipitation experiment Chromatin Immunoprecipitation Assay, Chip is a method of studying DNA and protein interactions in vivo environments, commonly used Study on the in vivo transcription factor binding sites and the like. The basic principle is to fix the protein-DNA...