JRD553 Immunuscularization (CO-IP) experimental histology

Experimental materials: protein

reagents, kits: Ripa Buffer PBS Protein A Agarose agarose ]

Instruments, Consumables: Centrifuge shaker EP tube cell scratch centrifugal tube culture plate electrophoresis Liquid chromatograph

experimental step

[ 123] 1, reagent preparation

1. Pre-cooling PBS, RIPA Buffer, cell scratch (after coating, buried in ice), centrifuge.

2. Two cells were washed twice with pre-cooled PBS and finally sucked PBS.

3. Add pre-cool Ripa buffer (1 mL / 107 cells, 10 cm petri dish or 150 cm2 culture flask, 0.5 mL / 5 u0026 Times; 106 cells, 6 cm culture Vegan, 75 cm2 culture flask).

4. Scrape the cells from petri dishes or culture bottles with pre-cooling cell scratches, transfer the suspension to 1.5EP, 4 ° C, slowly shake 15 min (EP tube interception, set the horizontal shaker).

The principle is that the cell is cleaved under transient conditions. At the time, many protein-proteins in intact cells are preserved. For example, use protein X antibody immunoprecipitates X, then the protein y in the body is also precipitated with X in the body. Currently, a refined Proreina is pre-cured on the beads of Argarose, enabling it with the antigen-containing solution and antibody reaction, and Proreina on the beads can adsorb the antigen to achieve refining purposes. This method is often used to determine both target eggs.Whitening is bonded in vivo; it can also be used to determine a new role of a particular protein.

The company is located at 7865 SW Nimbus Avenue (Space 29-G) Beaverton OR 97008, the laboratory area of u200b u200b600 square meters. The company currently has better biology, medical technology service platform, which provides customers with medical research sample testing, cooperative research, development and production services.

We have professional laboratories, advanced experimental equipment and experienced research technicians. Technical teams include two researchers (post-doctoral), 4 assistant researchers (Ph.D.), 15 core researchers (Master), mainly dedicated to genomics, transcriptional, proteomics, metabolic, biochemistry, pathological testing, Application and promotion of genetic testing in scientific research. Through highly subdivided, better service platforms, our customers provide comprehensive technical solutions and services. Currently, and, involving clinical medicine, tumor biology, immunology, cell biology, molecular biology, etc. more grateful is that the company has established an 1800 square meter animal center in 2015 to provide customers with more professional experimental animal breeding and modeling services. Time 荏苒, Perfect technical solutions and services.