Cell sorting experiment
Flow cytometry is based on flow cytometry, physical phragens , Physiology, biochemical, immunization, genetic, molecular biological character and functional state, etc. The cells are identified, classified, quantitatively and separated. The immunomagnetic bead cell sisdom is specifically labeled with super-magnetic MACS MicrobeADS (MACS miniature magnetic beads). After the magnetic label is finished, these cells are placed in a strong and stabilized magnetic field. The matrix in the selection column causes a high gradient magnetic field. The magnetically labeled cells remain in the column and the non-labeled cells flow out. When the split column removes the magnetic field, the magnetic labeling cells within the column can be eluted so that two cell components that are labeled and unmarked can be obtained.
Flow [123
(1)Take 1 cell
The cells to be detected, add 5 ml DPBS wash, centrifuge at room temperature 2000 r / min for 5 min, discard it, then use 200 u0026 MU; L 0.5% BSA-DPBS resuspension cells; thinking in cell suspension A fluorescent dye-labeled antibody, 4 incubated for 30 min; [
Dyeted cells were washed twice with 0.5% BSA-DPBS, then resuspended with 500 u0026 mu; L 0.5% BSA-DPBs. (4) Results statistical analysis. Method
[Centrifugation is collected to be separated from the cells, incubation with a small amount of PBE (0.5% BSA, 0.08% EDTA, pH 7.2), vacuum filtration and sterilization and liquid intracellular gas, sufficiently suspension (0.5 mL / 1 and liquid [123 Cell), added to the primary antibody (10-20 u0026 mu; g / ml final concentration), 4
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(2) [ 123] PBE was washed once with 20x volumes, and PBE (0.3 mL / 1 / mL
Cell After adequate suspension of the cell, the supermagnetic beads of the corresponding secondary antibodies were added, and the post-mixed posts 8 to 15 ° C [123 [] mount the separation column into the magnetic field, 0.5 ml of PBE was added, and it was naturally flowed under gravity to prepare the separation column. Service Description
(1) [ 123] The customer is provided: the cells are selected to be detected, and the cell species are selected. (2) Company provides: Basic experimental steps, pictures, data analysis results [123 ] . 2-3W, the specifically needs according to cell growth and experimental content Certainly.