Pathological Experiment HE Dyeing
Service introduction [ 123] The nature of easy being alkaline or acidic dyes is called basophysical and eosinophils, and the phenomenon of affinity for basic dyes and acidic dyes is called neutral. Cell acidic substances such as nuclear neat nucleus, adenocytes and neurocytes, the transparent cartilage matrix, etc., are stained with alkaline dyes, which are called basophilicity. Other proteins in cytoplasm, such as hemoglobin, eosinophilic particles and collagen fibers and muscle fibers, such as hemoglobin, and muscle fibers, such as acidic dye, referred to as acid dye. The phosphate group on the two strands of DNA (DNA) is outward, with a negative charge, acidic, and it is easy to bind to ionic bonds with a positively charged huper alkaline dye. Sumu is blue in the alkaline solution, so the nucleus is dyed in blue. Ingo is a chemically synthetic acid dye that is separated into negative charge in water, combined with the amino positive charge of the protein to binds cytosols, cytoplasm, red blood cells, muscles, connective tissue, Emoy, etc. Dedicated to varying degrees of red or pink, stark contrast to the blue nucleus.
Experimental process
(1) Put the slices of the dewaxed to water into the Sumujing water solution.
(2) a mixture of acid water and ammonia water, several seconds.(3) Flow water washed 1 h after the distilled water.
(4) 70% and 90% alcohol were dehydrated for 10 min.(5) Alcohol is red staining 2-3 min.
(6) Dye-stained slices were passed through pure alcohol, and then the slices were transparent to the xylene.(7) transparent slice is dropped on the Canadian gum, covering the cover slide.
Service Description[123 (1) The customer provides: paraffin specimens.
(2) Company provides: basic experimental steps, pictures, data analysis results. (3) Experimental period: 2 weeks, specifically, according to experimental content.