Pathological Experiment Day Wolf Star Red Dye

Tianyou Star Red is a strong acidic anion dye Each molecule contains 6 sulfonic acid groups, which is easy to bind to the alkaline amino acid group of collagen. After dyeing, it is not faded, and the dye molecule is long-shaped, and the collagen is arranged in parallel. . When there is a polarized light irradiation, the Nianmi Star Red and the collagen fiber reaction causes the collagen fiber to produce a significant double-variable light. Different collagen fibers have different optical rotation, exhibiting different colors under polarizing light microscope, borrowed to distinguish

[123 II , [ and [ Collagen, I [ Type fibers: tightly arranged strong double refueling, yellow or red fibers; Type fibers: show weak double refueling, a variety of loose mesh distribution; Type fibers: show weak buses, green fibers; [ Type fibers: showing weak double-crossed base membranes, pale yellow. Experimental process (1) The tissue is fixed to 4% polymethylene solution to prepare paraffin slices. (2) After slice deprnowled to water, the dimension of the dimension of Nianfang red staining 1 h. [123 (3) Tap water is slightly washed, wash away excess dye. (4) Sumu hydrocycloths were carried out 8-10 min, and washed 10 min in water. (5) Dehydration 30 S in anhydrous ethanol I, dehydrated in anhydrous ethanol II, 3-5 min. (6) transparent xylene, neutral gum sealing.

(7) Dyeing results were observed under polarized light microscope.

Service Description

(1) The customer provides: paraffin slice.

(2) Company provides: basic experimental steps, pictures, data analysis results.

(3) Experiment cycle: 2 weeks.