JRD613 paraffin embedded

1, paraffin embedded pathogenic service - dehydration
Alcohol is a commonly used reagent, can be solubilized with water, alcohol's dehydration ability, obvious Contract. Therefore, when alcohol is used as a dehydrator, it should be initially increased from low concentration alcohol, which can avoid overexeployment of tissue. At present, some dewatering machines have shocking functions, so that the dehydration process can be accelerated.
2, paraffin-embedded pathogenesis - Transparent
After tissue dehydration, the process of immersion wax is transparent, so that paraffin penetrates into the tissue to achieve embedding support. Since the dehydrating agents such as alcohol and paraffin are insoluble, use a medium that is alcoholic and paraffin, alcohol is replaced, and after alcohol is replaced, the organisms become transparent, that is, transparent.
3, paraffin-embedded pathogenic service - Dipwashaw wax
Tissue can be immersed in dissolved paraffin for soaking wax, paraffin gradually immersed in tissue gap, reflecting transparers, this process Take the wax. The quality and melting of paraffin are closely related to the sections, and the melting point and hardness of the paraffin used.
4, - Bulcted
Because, it is to remove the tissue block of the elixably from the dissolved paraffin, put into the embedding frame filled with molten paraffin, so that the tissue and embedding The process of reagent to phase melting and rapidly cooling. After the embedder is solidified, the organizational hardness and toughness are enhanced, thereby facilitating the slice.
5, - paraffin slice
After the embedding, you can make a slice. The embedded paraffin is mounted on the slicer, which is fixed, usually the thickness of the slice is 3-5 um, pay attention to the slice speed should not be too fast, preventing the slice of slice from being uniform. Chopped slices are attached to the slide, generally using the fishing method, but the slice often has a small horizontal pattern, and it can be attached after the exhibition, otherwise it affects dyeing and observation.
tissue sections are colorless and transparent, after dyeing, various fine structures can be observed, and the staining slices must be dehydrated, transparent, neutral resin seals, and then solidified Become a successful paraffin slice.