RNA interference experiment

RNA interference ( RNA interfering, RNAi) phenomenon refers to a silencer process after genetically transcribed with a target gene sequence homologous double-stranded RNA (DSRNA) bows, small interference RNA Specific mediated mRNA degradation of the same sequence, blocking the corresponding gene expression. The RAN interference technique provided by Crystal Libranes includes chemical synthesis small RN sections ( siRNA) and two forms of construct carrier RNA ( snRNA). The experimental client only needs to provide an intervention gene name or gene sequence D, design a suitable intervention site to ensure at least- - to effectively inhibit the gene expression of small RNA effectively, inhibitivity is not less than 70%.

RNA interference ( siRNA experiment) Process
determined interfering genes, designed and synthesized suitable small interference RNA (fragment or carrier type small interference RNA).
Pre-transfer experiments, cell culture, exploiting transfection conditions, time and necessary detection (WB, PCR, etc.), determined a pair of small RNAs with intervention effects.
The third step official experiment, set reasonable experimental grouping, instantaneous or stable transfection.
After the fourth step transfection, the cell biological test, protein detection, molecular biological detection, etc. were selected according to experimental requirements. The effects of small interference RNA for cells, proteins or gene functions. RNA Interference ( siRNA Experiment) Detection (can be selected according to the principal experiment requirements)
1. Cell testing: cell proliferation and toxic MTT, apoptosis, cell cycle, cell migration, cell invasion;
2. Protein detection: immunoblot Wb, immunocytochemistry ICC, immunofluorescence IF, flow cytometry Fcm, enzyme-linked immunization EL SIA;
3. Molecular biological detection: RT-PCR, real-time fluorescent PCR, Methylated specific PCR (MSP);
4. Other tests: biochemical testing, enzyme detection, lipid detection, sugar testing.