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Slow virus construction

Time:2018-02-20 Click:1119

Slow virus construct: Lentivirus is one of the reverse transcriptional virus, which has the basic structure and characteristics of reverse transcription virus; integrated into a host cell genome, long-term expression, can be prepared for transgenic animals; but there is also different from reverse The components and characteristics of the virus; more titers than the reverse transcription virus can not only infect split cells, but more importantly, it is possible to infect non-split cells.

Using L EntiVirus constructed of the SHRNA expression vector, as compared with the chemical synthetic siRNA and ShRNA constructed by instantaneous expression vectors, the Lentivirus-Shrna carrier is in the package system. After packaging, it can be used to infect cells that rely on traditional transfection reagents (such as primary cells, suspension cells, etc.), and can be whole u0026 nbsp after infection; in the genome of the sensing cells, for a long period of stable expression, It is a means of preparing stable expression of shRNA cell lines and in vivo experiments (in vivo).
At present, we can provide customers with one-stop service to construct from carriers to slow viral packaging purification and detection; the company also establishes and improves experimental techniques for re-transgenic animals in slow viral load based on high-efficiency and slow virus production technology. .

1. Discussion Analysis Experiment Scheme

2, determine service content
3, sign service contract
4, experiment, regular feedback
5 , Finishing experiment report
6, complete the contract content
7, follow-up experimental service, enter - step cooperation