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Molecular cloning experiment

Time:2017-09-01 Click:571

Introduction: Molecular cloning and plasmid vector construction service experimental technical services original authentic, quality assurance price concessions. Molecular cloning and plasmid vector construction services | Experimental technical services a PCZN1 plasmid + Arctic Express Host bacteria low temperature expression system.
We have a wealth of protein expression design experience and experimental operation skills, and the customer is not successful. All of our experimental data is truly believed, and we offer the original expression strain and cloned plasmid, and customers can repeat our experimental results based on our experiment report.
Technical Services:
Molecular Cloning and Plasmid Series Services | Experimental Technical Services
Sequencing Experimental Process:
Molecular Cloning Related carrier: DNA segment clone requires a suitable carrier, carrier or Plasmids, or phage, or viruses, usually mostly manually modified. As the carrier must have two conditions: - is that the carrier must be self-replicated in the cells, that is, the origin must have a copy origin; second, the carrier must have a suitable enzyme cut site, and these enzyme cut sites are not replicating the origin area Inside. The above two guarantees the reproductiveness and availability of the carrier. In order to facilitate the positivelonal clone, the carrier is often screening markers, such as the resistance of anti-bacteria, the color reaction of certain gene products, and the like.

Service Requirements:

1, please let us know the main purpose and specific requirements of our service.
2. Provide source and background information for exogenous DNA. DNA sources include: PCR amplification products: RT- PCR amplification product; DNA segment from other plasmids.
3. Provide electrophoresis photos of DNA segments and indicate that Marker has concentration.
4. Provide a vector source and background information.
5, indicating whether the enzyme cut site used and whether a flat end is required.