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Agarose gel electrophoresis experiment

Time:2020-10-30 Click:368

Agarose gel electrophoresis: techniques such as agarose gel as media DNA in an external electric field.

Experimental step
Gelatin: Use electrophoretic buffer to formulate 1.0% agarose gel
mixed by electrophoretic buffer: 2 u0026 mu; l - like buffer, 2 u0026 mu; l nucleic acid fluorescenceDyes, 10 u0026 Mu; LPCR product Mix
Draw: 10 children mixed onto gel pores
Electrophoresis: 90V voltage under electrophoresis 10 ~ 20min

u0026 nbspExperiment cycle and delivery standards

1. Experiment cycle: 1-2 weeks
2 Delivery standard: primer sequence, electrophoresis, original film, experimental report (experimental step, reagent, instrument u003e
Confirmation information
1, destination gene ID number and seed
2, sample type (tissue or cell, etc.)
3, sample nutrient
Charge standard service cycle provides results:
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