All gene synthesis experiment

The laboratory usually uses PCR and RT- PCR methods to acquire a gene fragment, but it is often unavailable because of specimens and templates, including
. Today, we use chemical synthesis to artificially construct a gene fragment, namely: all gene synthesis splicing technology. Can serve the majority of research friends.
Gene synthesis refers to a technique of synthesizing double-stranded DNA molecules in vitro, which is different from the oligonucleotide synthesis, and the oligonucleotide is a single-stranded, and the long flap can be only 100NT, which is only 100NT, and the gene Synthesis is a double-stranded DNA molecule synthesis, and the length range 50bp-12 KKB can be synthesized. According to customer needs, our company can provide all genetic synthesis services, fast speed, short cycle, price concessions. The synthetic gene can be connected to a different carrier according to the needs of the customer, and the products that end-be provided include strains and the complete sequencing report of lyophilized plasmids and all gene.
Scope of application of all gene synthesis:
1, the genes that cannot be obtained in conventional experimental means in nature.
2, researchers designed gene sequences through their own will to obtain genes that do not exist in nature.
3, the known gene sequence is optimized for rearrangement of the large mile codon to achieve efficient expression of the gene or obtain new biological characteristics.

1, the synthetic gene has reached 10.5 kB, and the gene has more than 10,000 genes.
2, can synthesize repeating sequences (no restrictions in repetition) high GC (less than 80%) hairpin (with reference to each 200bp less than 3 hair clips) Continuous single base repetition (GC is less than
15 AT Small than 20-rich genes, such as special structural synthesis, related data for genetic synthesis is based on
summarized according to the gene sequences that have been successfully synthesized by our company, to determine whether it can be smooth as you design gene sequence Synthesis As a reference specifically, it is necessary to be determined by the actual gene sequence.
3, genes can be cloned to the expression vector provided or expressed.
4, free genetic structure design genetic codon optimization gene cloning expression plan design and other genetic synthesis related consulting services.
All gene synthesis operation procedures:
1, we will analyze the genetic sequences you need to synthesize, check whether the genes are contained in the sequence and then design the synthesis
according to the specific situation of the sequence.
2, after getting you recognized, we will immediately perform single-stranded 0ligo DNA synthesis, DNA segment stitching and other work. The full-length gene was then cloned into the vector, and the correctness of the synthetic gene was confirmed by
DNA sequencing.
3, sequencing results If there is an error bit, we will take the corresponding technical means to repair the school, to ensure the correctness of the entire sequence.
Note: Due to the complex gene structure, notSome fragments can be successfully synthesized.For longer segments or some complex structures such as repeat sequences, high GC fragments, etc.,
can require additional time adjustment.If this happens, the technician will contact you in time to inform you of the synthesis process.The synthesized gene-general
is cloned into a universal vector, and if it is cloned in the customer's carrier, our company will charge a certain fee as appropriate, and the delivery time will be appropriately extended.