ITRAQ quantitative proteomics

ITRAQ quantitative proteomics Like the technical principles of ITRAQ and TMT, the molecular structure of the two is difference. Compared to SiLAC (labeled protein), ITRAQ and TMT were labeled with peptides. According to the quality of the ITRAQ / TMT tag, the relative quantification of the protein in the multi-set sample is achieved by MS:

SiLAC is quantified by primary MS (MSL), and ITRAQ / TMT is through secondary MS. (MS / MS.ms2) Perform quantitative ITRAQ / TMT can be compatible with source protein samples, and can simultaneously analyze samples of 8-10 groups, and analyze high volume.

iTraq quantitative proteomics

] 3, using different mass numbers of ITRAQ / TMT tags, respectively, the peptide samples are respectively labeled:

4, after the marking is completed, the sample is mixed, and the component separation is performed with SCX (15-20 components); 5 LC-MS analysis for each component:
6. Protein identification and quantitative analysis, screening differentially expressed protein;
7, biological experiment verification (Western Blot, EUSA, etc.):
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Technical advantages
1, high flux can simultaneously carry out the qualitative and quantitative analysis of the 8-10 sets of sample protein groups;
2, compatible Source samples (moving Plant tissue, bacteria, blood, etc.); 3, short experiment cycle.

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