1. IHC experimental test introduction
Tissue and cellularity of tissue sections or cell specimens in tissue sections or cell specimens , Positioning or quantitative research, this technique is called Immunhistochemistry or Immunocytochemistry technology. The immunohistochemistry is characterized by strong specificity, accurate positioning, and thus it can be clearly related to cell positioning, subcellular positioning and multi-protein between the protein. Immunohistochemistry is widely used in medical applications, especially in clinical tumor diagnosis and identification diagnosis. It is applied to a differential diagnosis of low dividing or undifferentiated tumors, and the accuracy can reach 50% -75%.
According to the antigen antibody reaction and chemical color development principle, antigenic and one anti-combination of tissue sections or cell specimens, Then, the secondary anti-labeled biotin, fluorescein or the like is reacted, and the chemical component in the cell or tissue is displayed by coloring reaction or fluorescence, and the antigenic antibody that can be clearly seen under optical microscopy or fluorescence microscope. The reaction product is capable of determining the distribution and content of certain chemical components on the cell climb or tissue slice.
Shiwax slice production;
Defaxing and hydration of paraffin slices
antigen repair;
Closed non-special Albty protein;
has biotin-labeled bifurcation incubation;
SP (Streptaviarin-peroxidase) reaction;
DAB color developed;
Reset, dehydration, transparent, and sealing.
Mirror test, take pictures.
Four, customers provide
tissue or cells, one anti-(available)
V. Company Provide
The test step, the result picture, the results analysis.
Experiment cycle: 1-2 weeks.