Quantitative protein group experiment
Quantitative protein syntax experiment service background description: SiL (Stable Isotope u0026 NBSP Labeling With Amino Acids In Cell Culture) Technology is based on the dependent principle of the essential amino acid according to the cell growth, using the essential amino acid of the isotope label (13C, 15N, 2H) to the cell protein group to make metabolic labeling; 13C 15N, 2H is a stable isotope, no radioactivity, and thus can be tested under conventional conditions. Quantitative protein group experimental service
Arg (r), Lys (k), Leu (L) is SiLAC Commonly used amino acids. R, K, L of (13C, 15N, 2H), such as R, K, and L is added to the cell culture medium, and when the cell is synthesized, these stable isotope labeled amino acids are naturally u0026 ldquo; incorporate u0026 rDQUO; into the protein. Through u0026 gt; 6 generation of cell proliferation, the protein group of the entire cell is stabilized in isotope amino acid u0026 rdquo; marker u0026 rdquo ;; SiLAC u0026 ldquo; marker u0026 rdquo; protein and u0026 ldquo; unmarked u0026 rDQUO The number of proteins (normal medium culture) has the number of quality differences, which exhibits a pair of bispressor peaks in one
stage mass spectrometry (MSL) of the subsequent LC-MS, by mass spectrometry peak The signal strength enables relative quantification of each protein expression level in the 2 group samples, Finance to determine protein expression level difference (differential proteomics analysis) and distinctive specificity and non-specific interaction (protein) Analysis of interaction); simultaneous pass The secondary mass spectrometry (MS / MS, MS2) is realized to identify the protein in the sample. u0026 Nbsp; SiLAC Technology Combined with LC-MS at the same time to achieve the qualitative and quantitative analysis of proteins in 2 groups. Experimental technology u0026 ldquo; high, large, upper u0026 ldquo; quantification, passing through the ink information can directly reflect the difference in protein expression between the samples, easy to quickly find the target molecule of interest. At the same time, using SiLAC technology, it can significantly improve the article .