TMT Mechanics Technical Service

TMT Hydrodynamics Technical Services

Simple introduction: Itraq and TMT technical principles, the different molecular structure is different. Compared to SiLAC (labeled protein), ITRAQ and TMT were labeled with peptides. According to the mass difference between the ITRAQ / TMT tag, the protein in multiple sets of samples is performed by MS, and the SiLAC is quantified by the primary MS (MSL), and the ITRAQ / TMT is passed. The secondary MS (MS / MS.ms2) is quantified J ITRAQ / TMT can be compatible with source protein samples and can simultaneously analyze the samples of 8-10 groups, and analyze the flux.

TMT method technical service experiment step: 1, extracting proteins and quantifies; 2, Trypsin is enzymatically hydrolysis, extracting the protein, and extracts the peptide; [ 123]

3, the peptide samples were labeled with different mass numbers:

4, after the label is completed, the sample is mixed, and the component separation is performed with SCX (15 -20 component); 5, LC-MS analysis is performed on each component: 6, protein identification and quantitative analysis, screening differentially expressed protein
7, biological experiment verification (Western blot, EUSA, etc.):

Technical advantage 1. High flux, the qualitative and quantitative analysis of up to 8-10 groups of sample protein groups at the same time; 2, compatible sources (animal and plant tissue, bacteria, blood, etc.); u0026 nbsp
3, short experiment cycle.