EMSA Homology Technical Services

EMSA Hydrodynamics

Gel migration or electrophoretic mobility detection is a technique for detecting proteins and DNA sequences, initially for studying DNA binding Protein and its associated DNA binding sequence interacts for qualitative and quantitative analysis. Divided into two types: isotope tag probe, non-isotope marker probe.

The purified protein and cell coarse extracts and 32P isotope labeled DNA or RNA probes are typically thermally enhanced, and isolated on non-denaturing polypropylene gel electrophoresis. Composite and non-binding probes. DNA-composites or RNA-composites are slower than non-binding probes. The isotope labeled probe is different from the binding protein of the study, but the double-strand or a single strand. When the DNA binding protein such as transcriptional regulators is detected, a purified protein, a partially purified protein, or a nuclear cytokinetic liquid can be used. When the RNA binding protein is detected, the protein can be extracted or partially purified according to the position of the RNA binding protein. DNA or RNA and oligonucleotide sequences (specific) containing protein binding sequences, and other non-related sequences (non-specific) are used to determine the specificity of DNA or RNA binding proteins in a competitive experiment. Specific binding is determined based on the characteristics and strength of the complex in the presence of competition.

Step: The label of the probe, purification of probes, formulation of EMSA glue, EMSA combination reaction, probe cold competition, mutation Needle cold competition, super-shift reaction, electrophoresis.

Content: The electronic measuring film result and grayscale value analysis, each film can be done 1-7 samples.