First, the primer synthesis service introduction

Primer synthesis is a perfect process of the OD value of the primer, dissolved primer, and ends into a perfect process of primers. At present, the primer synthesis is basically used to use solid-phase phosphormide trilamp. The phosphoride trilanside synthesis DNA segment, with efficient, rapid coupling, and stable starting reactants. The phosphoride trilanside method is to secure the DNA in the solid phase vector. The synthesis of the DNA chain is completed, the synthetic direction is synthesized from the 3-terminal portion of the primer to be synthesized, adjacent nucleotides pass 3 u0026 # 39; - u0026 rar; 5 phosphate bond.
Second, the primer synthesis service content

1, the
1
1 -base 5 -OH group u0026 nbsp is removed from the CPG monomer. The protecting group (DMTR) is ready to attach the next new base. 2. Activized new base monomer (phosphoramidite), preparation is reacted with an base. 3, the second base is coupled to the first base.
4, the No. 5 -OH plus caps of the first base group will not be reacted, which is no longer further involved in the reaction.
5, oxidation of nucleoside in oxidation into a more stable nucleoside phosphate (i.e., trivalent phosphorus oxidation into a five-vivo phosphorus).
6. Repeat one to five cycles until the desired primer DNA sequence is completed.
7. After the synthesis is completed, the primer molecule is cut from the CPG, and the purification of the in-step.
Third, the primer synthesis service description

1, can provide sequence or notification primer name

2, provide RT- PCR test service

3. If you have any questions, we will serve you wholeheartedly.
Fourth, quality commitment 1, primer freeze-dried powder; 2. Test report