RNA extraction

First, the RNA extraction service introduction

Trizol reagents are main components of isolal oxide oxide and phenol, of which isocholar oxide oxide lysate to lysis of cells to promote the dissociation of nuclear proteins, fast IMA and heavy whiteness are degenerated and put R14 peaks into the liquid. When hydrogen is added, it can be taken into acidic sample, and the acid phenol can promote XA into the aqueous phase, and the aqueous phase layer and the organic layer can be formed so that the weight is still left in the organic phase. White matter 0 and 1 n were separated. The aqueous phase (colorless) is mainly RIA, the organic layer (yellow) is mainly NA and protein.
Second, RNA Extraction Service

Total RNA extraction (TIZO | Extraction) Experimental step

After collecting bioma, RIA preparation is performed. If you need to temporarily store, you should take the rapid frozen biomaterials as soon as the Tao, and the bank is stored in the 50 cooling cabinet. When preparing a preparation U, the mass of the freezer will be exported, and the dance is broken from the addition of the Qing seeding. It cannot be thawed first to avoid RNASE.

When using me more than tissue, each 0-10m tissue side RNO trial is cleaved, and when CCI CCIDIT is 1, the first quarter is precipitated, each 5-10 x106 cells After adding 1 mi: THz0, it is repeated with a gun to blow or dramatically oscillate to lycodecell;

2 The 120-release transfer transfer of the above tissue or cells is placed under weight 15-0C,

3, in the above EP tube, add 2M chlorine every 1 ml Trizol, add chlorine, imitation, cover the EP pipe cover, in the hand is required for 15 seconds, at room temperature (150) - 30) After 2 ~ 3 minutes, 1200 (20 ~ -80) is a high heart for 15 minutes,

1. Upper water phase dispense in the new EP according to each M TrzoCl plus SM The amount of propanol was added to premium-deficit, and 10 minutes 12000 (2c ~ 8c) was placed at a weight (2c ~ 8c) for 10 minutes;

5, discarded, according to each 1mi Trizol plus 1 ml 75% ethanol for washing sea, vortex mix, 7500 (20 ~ 8C) centrifuge for 5 minutes, abandon _ supernatant;

6, let the precipitated RNA is naturally dry at room temperature;

7, use RNase-Free Water dissolves RNA precipitation.
3, RNA Extraction Service Description

1, customers provide: cells or tissues to be extracted;

2, Experimental cycle: 1 week;
[ 123] 3, if you have any questions, please consult our company, weWill be happy to serve you.