Comet experiment

First, the comet experiment service introduction

Comet experiments are also known as single cell gel electrophoresis experiments, which effectively detect the degree of damage to DNA chains in cells. The damaged DNA is migrated into the positive electrode during electrophoresis and forms a comet u0026 ldquo; the non-injured DNA remains spherical. The more severe DNA damage, the more DNAs migrated under the same electrophoretic conditions, the longer the corresponding migration distance. By measuring the optical density or migration length of the DNA migration portion, the degree of DNA damage of a single cell can be calculated, thereby further determining the relationship between the subject and the DNA damage effect.

Second, Comet Experimental Service

1 ] 3, DNA alkali;

4, single cell electrophoresis;

5, neutralization and dyeing;

6, experimental results observation and data analysis processing, finishing Report

Third, Comet Experimental Service Description

1, Cell Sample: Each test sample needs to provide at least 5 * 10 ^ 5 cells

2, fresh tissue samples, It takes 24 hours, 4c transportation to the laboratory, dry ice transport, will cause certain damage to the tissue and may affect the results.
3, if you have any questions, please consult our company, we will be happy to serve you.