Luciferase reporter gene detection

1. Luciferase reporter gene detection service introduction

Luciferase reporter gene system is a luciferin as a substrate to detect the activity of firefly fluorescene (Ireflyluciferase) activity Reporting system. Luciferase can catalyze Luciferin oxidation into Xyluciferin, in the process of Luciferin oxidation, Bioluminescence, can detect biofluorescence released during Luciferin oxidation by fluorescence assay equipment, often applied to start Research on Sub Transcription Activity Regulation and MiRNA Target Gene Verification.
Second, Luciferase Report Gene Testing Service

1, synthesis or amplification of the target fragment and subcloned to the target fluorescene reporter gene carrier;

2. Construct a transcription factor expression vector or synthesis corresponding MIMICSL, all of which are provided corresponding to the corresponding control;

3, the culture purpose cells are inoculated into the orifice plate, and the gene plasmid and transcription factor expression plasmid or corresponding MIMICS co-transfection cell. 4, luciferase detection fluorescence intensity and compare with no-load control;

5, analyze data and organize complete experiment report

Third, luciferase report gene detection service description

1, promoter transcriptional activity regulation: Provide transcription factor name and ID, target gene name and ID; 2, miRNA target gene verification: provide miRNA name, species information, target gene name and ID; 3, stay Testable cells; if there is no special requirement 293T cells;

4, if there is a genetode template or constructing a good transcription factor expression vector,

5 can be provided, if you have any questions, welcome We will serve you wholeheartedly.