Carrier construction
First, the carrier construction service introduction
vectorconstruction is transported to the receptor cells in the receptor cell. First, it is necessary to find a carrier that can enter the cells that can still be copied in the form of a foreign DNA section. The ideal carrier is a plasmid, in the genetic engineering. Medium, commonly used plasmid as a carrier. When the plasmid is inserted into a segment of the exogenous DNA, it is still able to copy itself. Carrier construction is one of the common means of molecular biology research. u0026 Nbsp;
2, TA clone
3, connected to the expression vector
4, transform Escherichia coli
5, sequencing identification
Third, carrier construction service description
1, customer provides experimental scheme required for carrier construction and transformation, destination gene product And sequences (if the purpose of the purpose of genes is not well prepared)
2, if the customer only provides samples such as organizational, cells, also need to provide a target gene NCB login number, and the company will additionally charge the RNA extraction and reverse transcription cost.
3, for the construction of the expression vector, report gene carrier, and viral vectors, you need to provide sequence information, desirable to clone to the carrier, enzyme dug bit, etc.;