Scanning electron microscope

First, the scanning electron microscope service introduction

Scanning electron microscope, ie scanning electron microscope (SEM) , the sample is scanned by extremely narrow electron beam, and the electron beam and the sample are used. J uses a variety of effects, that is, the surface morphology of the sample is observed using secondary electronic signal imaging, often used for observation of the surface of the material and the sectional stereoscopic structure.

Second, scanning electron microscopy service content

1, cleaning: - - Ph7 2 phosphate buffer

2, fixed: Conventional Law 2 u0026 nbsp 5% WU diadelede fixed 24h (put into 0- 4C refrigerator). Some special samples, such as young, high water content, fixed 2 h after four yang, sodium, 4H

3, dehydration: ethanol series 30% - 50% -70% u0026 nbsp ; -85% - 95% - 100% (2 times) step-by-step dehydration, 10-15 min per level, blocks should be properly shaken, guarantee dehydration clean

4, intermediate fluid exchange: two steps. Type acetate (alcohol) WU ester: ethanol u003d 1: 1 mixture for 10 min, the second step is solened to be soaked for 10 min, appropriately shake

5, the critical point dry: generation After transferring samples into the sample basket, put it in the pre-cooling critical point dryer sample chamber, cover the liquid carbon dioxide after the chamber, to submerge the sample, first temperature rise to 15 it heaters 10 min, then warm to 35C It is gasified, and the liquid is slowly released. It must be placed to be allowed to open the storage

6, paste the sample: - Type sample can be pasted with double-sided tape, if the sample block is large , The conductivity is poor, the electrical adhesive must be pasted

7, the silicon retraction mirror observation

Third, the scanning electron microscope service description

Send sample requirements:
[ 123] Tissue sample

Requirements: Cut the tissue into 1 cubic millimeter (scanning electron microscopy, slightly greater than this standard) is fixed to 2. 5% wu dialdehyde fixation

Transport: 4 degree ice bag transport;



Cells directly scraped, cell suspension is directly centrifuged, discarded, PBS washed 2 times, centrifugal, Fixed 2.5% WU dialdal fixed liquid

Transportation: 4 degree ice bag transport;

Microbial bacterial sample

Refer to cell sample, the bacterial liquid is fixed after WY AldehydeLiquid, 4 degrees transport;