ROS active oxygen detection experiment

1. ROS detection service introduction

Active oxygen ( reactive oxidative species, ros) refers to oxygen-containing free radicals related to oxygen metabolism in biology. The intermediate product having high activity in the form of the base form can cause oxidation of the cell membrane structure, nucleic acid and protein molecules, causing structural and dysfunction, and ROS increases the body oxidative stress response (Oxidative stress) causes cell damage, participation Pathological mechanism. The ROS content of the cells can be detected, and ROS can be understood in the cells in the cells changes in the condition and pathological state.
Second, ROS detection service content

The choice of experimental method

1, the fluorescence microscope directly observes the photo, providing original fluorescent pictures;

2, utilization The fluoresceransseraser is detected, reading OD value results;

3, flow cytometry detection, outflow diagram;

experimental process.

1, collect The cell culture plate is adjusted to the cell suspension;

2, according to the experimental conditions, the processes are disposed, and the probe is loaded for ROS detection;

3, according to the experimental requirements, fluorescence The enzyme label, flow cytometer or fluorescence microscope detected and analyzed the results of the experiment.
Third, ROS detection service description

1, tissue sample, cell sample to transport or add protein protective agent after drying;

2, cell samples can also Direct express delivery culture bottle (sealing guarantee is not polluting, the culture fluid does not leak, the cells do not grow too full, 50%, filled with culture) u0026 nbsp ;;

3, serum or supernatant Send ( Sealed).
4, if you have any questions, we will serve you wholeheartedly.