Transmission electron microscope

1. Transmission electron microscopy detection service introduction

Transmission electron microscope, ie transmit electron microscope (TEM) , the electron beam is a light source, it can be seen that it cannot be seen under optical microscope. 0. 2 um ultrastructure, mainly used in ultrastructural observation within the internal part of the substance.

Second, transmitting electron microscopy detection service content

1, tissue: tissue block less than 1 cubic millimeter;

2, fixed: 2.5% wu aldehyde, phosphate buffer The liquid is fixed for 2 hours or longer. 15 min, 3 times with 0.1 M rinse rhazing fluid. 1% squeezed acid fixation fixed 2-3H. Rinse 15 min, 3 times;

3, dehydration: 50% ethanol 15-20 min, 70% ethanol 15-20 min, 90% Z lysed 15 -20min; 90% ethanol 90% prop, ketone (1: 1) 15-20 min, 90% C , ketone 15-20 min is carried out in on the 4C refrigerator). 100% propanoc, ketone, room temperature 15-20 min, 3 times; 4, embedded: pure c, ketone + embedded liquid (2: 1) room temperature 3-4h; pure c, ketone + embedded liquid (1: 2), room temperature overnight; pure encapsulation 37C 2-3H; 5, curing: 37C oven overnight, 45C oven 12h, 60C oven 48h;

6, ultra-thin slices Slices of 70 nm u0026 nbsp ;;

7,3% acetate uranium citrate double staining;

8, transmission electron microscope observation, shot

Third, transmission electron microscope detection service

1, tissue sample

Tissue cut into 1 cubic millimeter is fixed to 2.5% glutae, dialdehyde fixation; transport: 4 degree ice bag transport; 2 Cell sample

The cells are directly scraped, the cell suspension is directly centrifuged, discarded, PBS washing 2 times, centrifugal into group, added 2.5% gluta, dialdalde fixed liquid;

Transportation: 4 degree ice bag transport;



Refer to cell sample, the bacterial liquid is fixed after centrifugation of gluta, dialdehyde solution, 4 degrees transport;
u0026 nbsp