Calcium ion detection

First, the calcium ion detection service introduction

Calcium ions play an important role in the physiological activities of cells, the changes of free calcium ion concentrations and cellular function, transmission of signals and their damage And we have close contacts. Calcium ions are a single cell survival and death signal, and regulate its function. Calcium flow detection is a method of screening of G protein coupled receptors (GPCR) in drug development, and changes in intracellular calcium ion concentration are also one of the means of studying the study of cytomy. therefore, calcium ion fluorescent dyes are widely used, which have cytotropic, so they can be dyed.

Second, calcium ion detection service content

1. Preparation of the dyeing working fluid

2 Collect the cultured cell sample to be tested, wash cells 3 times with HBSS [123
3. After centrifugation, the dyeing working fluid was added to the cells and incubated for 20-60 minutes at 37C 4. Wash the cells 2-3 times with HBSS

5. Resusted by HBSS. The 37C was incubated for 20-40 minutes

6. The cells were then used to detect fluorescent calcium ion detection

, the calcium ion detection service description

customer provides cells, or use Cells in our cell bank