Cell cycle detection

Cell cycle detection
u0026 ldquo; cell cycle also known as cell division cycle u0026 rDQUO; refers to a whole process of cells that have been born with born long, division, and generally can be divided For a number of phases, that is, the G1 period, the S phase, the G2 period and the M phase. The cells were prepared in G 1 completion of the necessary growth and substances, and the replication of its genetic material-chose DNA was completed in the S phase, the necessary inspections and repairs in G2 phase to ensure the accuracy of DNA copy, then in M Complete the allocation of genetic substances into child cells and make cells - - divided into two.
Cell cycle detection means
1. Cell counting method;
2. BRDU penetration method;
3. Flow cytometry (PI staining method) u0026 nbsp ;; 123] 4. Isotope labeling (marked with mitosis) u0026 nbsp ;;
5. Immunofluorescence staining [P27K and HCDT1 (30/120).
Common diseases related to cell cycle

Leukemia (one of leukemia may be is caused by hematopoietic stem cells from splitting normally, loss of blood loss)

kidney disease (many kidney disease, Especially those with inflammatory renal spheres and renal tubular diseases are accompanied by obvious cells. Groundbumin and kidney are small
tube-based cells proliferate and then promoted)
Dermatology (skin cell abnormal hyperplasia)
Several proteins (123] 1. periodic protein-dependent protein kinase ( CDK) : CDK) : CDK) : CDC2 and cell cycle protein binding The activity of kinases, sensitive cell cycle protein-dependent kinase
(Cyclin-Dependent Kinase, CDK).
2. Cydin u0026 nbsp ;: Participate in the cell cycle regulated protein, and its concentration is floating in the cell cycle. As
in the cell cycle stage, sometimes the concentration is as high as a thousand times, and sometimes it is reduced to zero. Periodic protein as - specifically subunit, combined with periodic protein-dependent protein kinase and activates
3. Periodic protein-dependent protein kinase inhibitor (CKI) u0026 nbsp ;: Enable combination with periodic protein-dependent kinase Suppressing proteins of their activity. Divided into
in mammals, Cip / Kip and Ink4 families.