ROS oxidative analysis
ROS oxidative analysis
1. ROS: is the mitochondria by the state II to the state IV conversion of high oxygen-free, highly reducing breathing chains to leak the large miles and reduce oxygen molecules to form, activityThe production and removal of oxygen
can often cause damage to the human body.
2. Detection method: fluorescent probe method
1. After taking the cells, PBS is washed to remove residual medium;
sub-testLiquid, 1H, oDCF + DA.37C cell culture tank, 1H.
3 was cultured with blood-free cell culture three times to sufficiently remove CHDAO
4. u0026 nbspPBS green wash twice, swappicted to collect cells, and fluorescence microscopic tract in fluorescent photo.
1. Experiment cycle: 2-3 weeks
2. Delivery standard: fluorescent picture, fluorescence value, experimental report (experimental step, reagent, instrument, etc.)
Information needs to be confirmed
1. Sample species and type (tissue or cells)
2. In the sample number
3. Whether to provide probes