JRD260ITRAQ quantitative proteomics

iTraq quantitative proteomics ITRAQ and TMT technical principles, Different are the molecular structure of the two. Compared to SiLAC (labeled protein), ITRAQ and TMT were labeled with peptides. According to the quality of the ITRAQ / TMT tag, the relative quantification of the protein in the multi-set sample is achieved by MS: SiLAC is quantified by primary MS (MSL), and ITRAQ / TMT is through secondary MS. (MS / MS.ms2) Perform quantitative ITRAQ / TMT can be compatible with source protein samples, and can simultaneously analyze samples of 8-10 groups, and analyze high volume.

ITRAQ quantitative proteomics
1, the protein is extracted and quantified; 2, Trypsin separately enzymatically enzymatically The peptide segment is extracted;

3, the peptide sample is labeled with different mass numbers:

4, after the marking is completed, the sample is mixed, and the component separation is performed with SCX (15-20 components)

5, LC-MS analysis is performed on each component:

6, protein identification and quantitative analysis, screening differentially expressed protein; 7, biological experiment verification (Western blot, EUSA, etc. :



Technical advantages
1, high flux, can simultaneously carry out up to 8-10 sets of sample protein groups Qualitative and quantitative analysis;
2, compatible sample (animal and plant tissue, bacteria, blood, etc.); 3, short experiment cycle.

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