JRD234ITRAQ quantitative protein group
iTraq quantitative protein group iTraq quantitative protein service advantages:
High sensitivity, low detection limit, can detect low abundance protein;
Strong separation ability, wide analysis range, Identification of type proteins can be isolated, including high molecular weight proteins, acid proteins and alkaline proteins, membrane proteins and insoluble proteins; high flux: analysis of 8 samples, improved experimental flux, but also Analysis of multiple time points or different processed proteins; Results Reliable: The qualitative and quantitative analysis results are more reliable; High degree of automation: liquid phase and mass spectrometry, automated operation, fast analysis speed, separation effect Okay.
Technical features of ITRAQ quantitative proteome:
High efficiency sample pre-processing: Perform high PH RP-HPLC efficient separation, automated collection uniformity. High Identification Rate: Systematically study tissue or cells as much protein as much as possible, can identify up to 6,000 proteins (with human HEPG2 as an example). Quantification: Each set can be made of 8 samples, and a single sample marking efficiency can reach 98%, 8 mark simultaneously, perform experimental errors caused by sample processing and batch mass spectrometry.
Excellent performance: AB EX 5600-PLUS is based on high resolution (~ 30000), high quality precision (less than 2 ppm) mass spectrometry platform, can achieve more accurate results. In addition, the instrument has no species specific limitation, theoretically useful for species. Service content and experimental process:
Sample preparation and quantification; Extract, ITRAQ reagent mark;
except salt;
isometric mixed labeling peptide;
MS / MS mass spectrometry detection and analysis;
related biological information analysis; Experiment report submit
Protein identification, quantification protein GO notes, Pathway Metabolic passage notes
Differential protein Go, Pathway function enrichment analysis
Technical repeatability analysis (items for repeated design)
multi-sample expression mode cluster (applicable to samples u0026 ge; 3 projects)
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Protein identification and quantitative analysis results; Customer protein corresponding to mass spectrometry; Complete experiment Step, use the instrument, software retrieval parameters, etc .;
Detailed data analysis results show
Customer Send sample notice:
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You only need to provide samples, there is no need to provide additional reagents; The amount of protein you provide u0026 ge; 50 ug, concentration u0026 ge; 5 ug / ul, otherwise the isotope cannot mark You can also choose to provide direct organization or cells, follow-up operations. Protein samples need to be sent with dry ice or liquid nitrogen, and customers in Shanghai can wait for them.
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