JRD229SiC quantitative protein group

We have a professional laboratory, advanced experimental equipment and experienced research technicians. Technical teams include two researchers (post-doctoral), 4 assistant researchers (Ph.D.), 15 core researchers (Master), mainly dedicated to genomics, transcriptional, proteomics, metabolic, biochemistry, pathological testing, Application and promotion of genetic testing in scientific research. Through highly subdivided, better service platforms, our customers provide comprehensive technical solutions and services. Currently, and, involving clinical medicine, tumor biology, immunology, cell biology, molecular biology, etc. More grateful is that the company has established a 1800 square meter animal center in 2015, which can provide more professional experimental animal breeding for our customers. And modeling services.

1. SiLAC quantitative proteomics principle: SiLAC ( Stable isotope labeling with amino acids in cell culture) technology Methods of metabolic labeling proteins designed for dependent principles of essential amino acids (Lys, Arg, etc.) by mammalian cells. Take LYS as an example, there are 6 12C atoms in ordinary Lys molecules, and use 13C to replace all 12C atoms in ordinary Lys, thereby generating 13C marked Lys. The 13C marked Lys is more than 6DA than ordinary Lys. For example, two groups of cells, a group of culture medium containing ordinary Lys (remembered K0), a group of Lys culture containing 13C (rejected K6). Since Lys is an essential amino acid of cell proliferation, the 13C-labeled Lys Natural u0026 ldquo; incorporates u0026 rDQUO; into the new and formed protein, the protein group of the entire cell is marked by the 13C labeled Lys. In view of the two amino acids of K0 and K6, there is a difference in mass of 6da, and when the protein is analyzed in subsequent LC-MS / MS, the identification and quantification of proteins in the sample can be completed simultaneously (6DA quality difference). According to the quantitative results, it is possible to directly determine the difference in protein expression, distinguishable specificity and non-specific interactions. Second, SiLAC quantitative proteomics characteristics:

(1) Advanced, performing cells protein interactions and quantitative proteomics technologies in protein expression differences;

(2) Analysis by LC-MS / MS, energy can be at the same time, it is achieved with protein molecules in unknown samples; identification and quantification; (3) in accordance with quantitative resultsIt can directly determine the difference in protein expression, distinguishing specificity and non-specific interactions.




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